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Beilstein J. Org. Chem. 2013, 9, 254–259, doi:10.3762/bjoc.9.30
Graphical Abstract
Figure 1: The chemical structure of a series of fluorescent amino acids. (1a) α-(2-(7-hydroxycoumarin-4-yl)et...
Scheme 1: Synthetic route to fluorescent amino acids 1a, 1b and 1c.
Figure 2: Coomassie-stained SDS-PAGE (left) of TAG38 mutant thioredoxin (indicated by the black arrow) expres...
Figure 3: Absorbance of compounds 1b and 1c at 360 nm as a function of pH value. (A) Absorption spectrum of 5...
Figure 4: Effect of the pH value of the solution on the fluorescence emission spectra of compounds 1b and 1c....